2.2. Rice bran preparation
Black glutinous rice bran (O. sativa L.) samples were purchased from
a rice milling factory (10% degree of milling) in Roi Et province,
Thailand. The fresh bran samples were prepared in triplicate. In total
there were 30 samples for 10 treatments including the raw bran. All
samples were immediately passed through a sieve (no. 20) to remove
broken pieces of rice and husks to obtain the rice bran fraction. The
initial moisture content of the rice bran was determined using the
AOAC (2000) method.