were 3rd generation
offspring
practices in the aquarium
facilities
Determination of the LD50 of the WSSV strains
All prawns injected with 10−1, 10−2 and 10−3 di -
lutions of WSSV-Thai-1 began to shown gross disease
signs from 24 hpi. Among prawns injected with dilutions
of 10−4, 10−5 and 10−6, only those in which infected
cells were evident when sampled at 120 hpi
showed disease signs from 24 hpi. Except for no white