2 and RSIV Namhae, respectively (Figs. 2 and 3). The homology of the partially
sequenced RNRS, ATPase, and DPOL genes of RSIV CH-1 using the amplicons produced
after PCR with the reported primers (Table 1) were 93.8%, 96%, and 98.3% compared
with those of RSIV Namhae, respectively (data not shown). In comparing the nucleotide
sequence corresponding to the 1F primer, there was only a single nucleotide difference at
the 18th position of the 1F region in the DNA of RSIV Sachun. However, RSIV CH-1
showed five nucleotide differences at the 3Vend of the 1F region, which is known to be an
important part of the primer for successful PCR amplification (Fig. 3). This five-nucleotide