2.3. Morpho-physiological studies
Morpho-physiological observations of the isolates were made from day 2 to day 6 of cultivation on PDA at 20 C or 25 C. Isolate identification was based on colony colour, pigmentation of reverse side and odour; sporulation type, conidia shape, colour, and dimension; growth rate and optimal growth temperature.
Growth rate of the isolates was defined by measuring colony diameter every day for 72 h.
The effect of temperature on growthwas studied on isolates grown on PDA in darkness at 15, 20, 25, 30 and 35 C for 4 days.