Scale-up strategies
The bioethanol production by E.coli KO11 was step-wise scaled up
from 100mL shaken flask to 10 L stirred-tank bioreactor under laboratory
conditions as shown in Fig. 1. The scale-up was conducted
from shaken flask to 2 L bioreactor using the constant Reynolds number.
For the stirred-tank bioreactors, the scale-up was performed
using the constant impeller tip speed as a scale-up methodology.
Mathematical equations of scale-up process for bioethanol production
are presented in Table 2. For stirred-tank bioreactors, the power
numbers were obtained froma Power number curve as a function of
the impeller Reynolds number and the impeller type (Geankoplis,
1983). The characteristic empirical constant (k) for a standard
Rushton turbine impeller was taken as