2.2. Immobilization and co-immobilization
S. cerevisiae was mixed with 2.5% Na-alginate solution
and the slurry culture was added dropwise into a 6% ) CaCl2
solution using a 50 mL syringe Once the slurry was added to
the solution, beads of Ca-alginate with entrapped cells were formed
with a mean diameter of 3e4 mm. For co-immobilization S. cerevisiae and A. oryzae or M purpureus were mixed together and immobilized as described above.