Cephalopods are well known for their secondary metabolites, but few studies have been carried
out on bioactive compounds of their cuttlebone. This study focused on the antimicrobial activity of the chitosan
and polysaccharide extracts from the cuttlebone of Sepia pharaonis against 8 bacteria and 2 fungal strains using
disc diffusion method and minimum inhibitory concentration (MIC) were also calculated. The amount of total
content of S.pharaonis polysaccharide, chitin and chitosan and was found 30%, 19% and 11% respectively.