This laboratory experiment allows students to create and test
their own columns for use on an HPLC instrument. All of the
student results were similar to those obtained by the lead author
(see the Supporting Information). Students experience researchlike
conditions, in which separations may or may not occur
according to what they predict or expect. These experiments are
aligned with previously published work calling for redesign of
analytical chemistry courses.39 This laboratory allows for a more
open-ended and research-like experience for analytical chemistry
students.