2.2. Adaptation of the strain
Progressive adaptation of Zymomonas mobilis in soybean
molasses was done through successive transferences from
the seed culture (10% inoculation rate). Initially, it was used
soybean molasses at 50 g L-1 of soluble solids (first transference), and thereafter (second and third transferences)
soybean molasses at 100 g L-1 of soluble solids. The stock
culture was stored for no longer than 3 weeks at 4 C. These
cultures were grown at the same conditions described
previously.