Considering the results obtained in inter-examiner reproducibility,
the protocol that presented the highest reliability to measure Pz-sap values was the one that used digital photographs and took measurements on a computer,
on a natural light background, and with measurements being carried out on triplicate colonies. This result reinforces the
importance of measuring the enzymatic activity of SAPs in triplicate.
It also reveals the importance of assessing the statistical calibration between the different researchers when taking the same measurement,
since only one of the eight protocols assessed showed data with sufficient reliability when four different examiners were involved in performing the analyses