Algae oil is a major source of n-3 PUFA and the main omega-3 fatty acid, DHA in base algae oil can reach to 50%. Unlike bulk vegetable oil, the water content in algae oil is extremely low (less than 50 ppm). The antioxidants applied in such oil include mixed tocopherol, ascorbyl palmitate, lecithin, and rosemary extract, all of which are hydrophobic antioxidants. The fate of hydrophilic antioxidants in such oil, however, is unpredictable so far. Therefore, the purpose of this research is to study the effectiveness of several popular hydrophilic antioxidants in the algae oil and water-in-algae oil emulsions. In addition, the role of emulsifiers on the chemical stability of water-in-algae oil emulsion will also be examined.