Serial dilutions were subsequently prepared with the
same diluent. Populations of B. animalis were enumerated by the
pour plating technique using 1 mL of each dilution in MRS-LP agar
(Oxoid, UK) followed by anaerobic incubation (Anaerobic System
Anaerogen, BBL, EUA) at 43 C for 72 h. The results were expressed
as log CFU/g and also as survival rate (%) according to Magarinos,
Selaive, Costa, Flores, and Pizarro (2007).
Serial dilutions were subsequently prepared with the
same diluent. Populations of B. animalis were enumerated by the
pour plating technique using 1 mL of each dilution in MRS-LP agar
(Oxoid, UK) followed by anaerobic incubation (Anaerobic System
Anaerogen, BBL, EUA) at 43 C for 72 h. The results were expressed
as log CFU/g and also as survival rate (%) according to Magarinos,
Selaive, Costa, Flores, and Pizarro (2007).
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