Physical and biochemical characterization of freeze-dried Aloe vera L. gel (AG) has been carried out from different age groups and harvesting seasons to get a clear scenario of AG properties at different growthperiods. The stability of amorphous AG under thermal perturbation was analyzed with reference to therespective glass transition temperature by differential scanning calorimetry. The functional properties ofgel in the form of water holding and fat adsorption capacity were more pronounced at 3-year-old plantsin summer. A higher glass transition temperature (135 ± 4.80◦C) of gel from 3-year-old aloe accountsfor higher concentration and better cross-linking of the component polysaccharides. The chemometricstudies encompassing principal component and partial least square analysis depict an intrinsic structure-function relation with reference to different properties considered, which has been demonstrated to beinfluenced by growth periods of plants.