The digested fragmentof bkt was ligated to the linearized pRT100 between the XhoIand XbaI sites. The cloned pRT100 was named as pRT100-BKTand it was further digested with the HindIII (A-AGCTT) to releasethe bkt fragment containing CaMV 35S promoter and the poly Asequence. Similarly, the binary vector pCAMBIA1304 that harborshygromycin phosphotransferase (hpt) as selectable marker geneand green fluorescence protein (gfp) and -glucuronidase (UidA)as reporter genes was also digested with HindIII.