Although no antimicrobial activity could be observed in the
diffusion assay for any active film, the dilution method clearly
evidenced that films containing 5% and 6.5% of Proallium® were
able to significantly reduce the growth of all organisms used from
the first day of contact. As observed in Table 4, the dilution method
used to quantify the bacterial reduction caused by films showed
decreases in microbial growth for all of the strains assayed when
films containing 5% and 6.5% of Proallium® were used. These decreases
were extremely significant for all microorganisms in contact
with these concentrations of the active film from the first day.
However, the growth of Staphylococcus aureus only decreased
significantly in the case of 6.5% Proallium® on the first day.