The effect of addition of macronutrients (Stock A) could be
determined when we compared the explant cultured in Media C
with those in control media (Media B). From Fig. 3, the growth
index of H. callitrichoides inoculated in Media C was significantly
greater than those in Media B (P < 0.05). The growth index of
explant increased significantly from 3.82
0.35 g/g to 7.14
0.88 g/
g when macronutrients (Stock A) was introduced in Media C.
Therefore, it could be seen that macronutrients such as potassium,
phosphate ions, nitrate ions and ammonia were important for the
propagation of H. callitrichoides [19]. In addition, the chlorotic
characteristics of leaves and necrosis that occurred in explant
cultured in control media (Media B) was not observed in the
explant inoculated in Media C as shown in Fig. 4 [20]. This is due to
the addition of macronutrients that had replenished the important
nutrients such as ammonia, magnesium, nitrate ions, potassium
and phosphate ions for a better propagation of H. calltrichoides.
These observations were in agreement with those reported by Taiz
et al. [21] that the deficiency in magnesium, potassium, nitrogen
and phosphorus could lead to the chlorosis and necrosis of the
tomato leaves.