The topology of the tree was in agreement with that of previous reports (Gundersen et al., 1994; Seemuller et al., 1994; Lee et al., 1998), indicating the equivalence of our approach with those generally accepted.
The Spanish faba bean sample clusters, with high confidence, with the 16S rIII group (Fig 2). A different tree prepared with only phytoplasma members of group 16S rIII showed that a group adscription placed our sample close to Chayote witches’broom phytoplasma (Fig 3).
The symptoms found in faba bean plants did not resemble those produced by virus of fungal diseases and no phytoplasmas had been previously reported on faba bean in Spain.Our results with graft transmission to periwinkle of faba bean showing symptoms, amplification by PCR from both faba bean and periwinkle plants and similar characterization of amplified products,