2.2.5. Percentage of arbuscular mycorrhizal (AM) fungal root
colonization and glomalin production
Intraradical arbuscular mycorrhizal (AM) fungal colonization
was assessed after clearing washed roots in 10% KOH and staining
with 0.05% trypan blue in lactic acid (v/v), according to Phillips and
Hayman (1970). The extent of mycorrhizal colonization was
calculated according to the gridline intersect method (Giovannetti
and Mosse, 1980).