discussionThe concentration of four monosaccharides and five inhibitorswere measured before and after cultivation to evaluate theconsumption of sugars and changes in the concentrations of fer-mentation inhibitors during cultivation. The concentration of fourmonosaccharides and five inhibitors were also measured for hotwater extraction before and after autoclaving. The analysis ofmonosaccharides is summarized in Table 2. The cultivations inwood hot water extract (HWE) without autoclaving did not resultin any substantial cellulose activity and this result was somewhatsurprising, since it is not clear that the media contained com-pounds that inhibit A. xylinus 23769. This could have occurred dueto contamination of the HWE, which was not sterilized. However,in the Uraki, Morito, Kishimoto, and Sano (2002) study, bacterialcellulose production was not observed in the unpurified water-soluble fraction (WS) of waste liquor from atmospheric acetic acidpulping due to presence of antibacterial activity in the unpurifiedWS. BC production was only observed after purifying the mediumwith activated carbon. Therefore, wood hot water extract shouldalso be studied in terms of antibacterial activity. Monosaccharideconcentration also did not change significantly before and afterautoclaving. Cultures at pH 5 and 26 and 30◦C and at pH 6 and26◦C did not produce BC and the reason why BC is not producedat these conditions requires further investigation. The results inTable 2 indicate that glucose and xylose were the main nutrientsources in all cultivations.