Semen samples that gave the success of getting full DNA profile rate 75% in all substrate test and the other 25% of the sample DNA profile is a high partial. Because of, this education not be an additional DTT in sample preparation step. (ได้ลองใส่ DTT แล้วเป็นไง บอกผลคร่าวๆ) Unable release DNA out as expected. Table 2 shown as the dilution ratio is proper of three biological samples.