used a pH regulated continuous culture (dilution rate controlled by
pH) with ethanol in the feed to select, within a few days, the most
tolerant strains among a mixture of Saccharomyces cerevisiaehybrid
strains. Dinh, Nagahisa, Hirasawa, Furusawa, and Shimizu (20 08)
also recovered ethanol adapted yeast strains from a haploid labo-ratory strain af ter a stepwise increase in ethanol concentration from
0 to 10% during repetitive cultivation. Stanley, Fraser, et al. (2010)
obtained mutants with increased growth rates in sub-lethal
ethanol concentrations by continuous culture of a haploid strain
in YEPD supplemented with increasing amounts of ethanol.