The samples of each stage of purification were analyzed by
SDS–PAGE (Fig. 2). The purified Gal-6-sulfurylase revealed a single
band indicating that the enzyme was purified to apparent homogeneity.
The molecular weight of the purified Gal-6-sulfurylase
was estimated to be 65 kDa, which is similar to the Gal-2,6-
sulfurylaseIfrom Chondrus crispus