The assay was based on the reduction of Mo (VI) to Mo (V)
and subsequent formation of a green phosphate/Mo(V) complex
in acid pH[13]. A total volume of 0.3 mL extract dissolved in
methanol was added to 3 mL of reagent solution (0.6 mol/L
sulphuric acid, 28 mmol/L sodium phosphate and 4 mmol/L
ammonium molybdate). The mixtures were incubated at 95 °C
for 90 min then cooled to room temperature. The absorbance was
measured at 695 nm. The total antioxidant activity was expressed
as the number of equivalence of ascorbic acid and BHT.