where A is optical density in each sample, a is length of light path in the cell (usually 1 cm), v is volume of the extract in ml and w is fresh weight of the sample in ‘g’.
Chlorophyll Stability Index (CSI) CSI in leaf tissues was estimated using a spectrophotometer following the method of Koleyoreas (1958). It was calculated as the difference in light transmission percentage between treated and untreated leaf samples at 652 nm.