Two hundred grams of milled coffee husk (0.4–0.8
mm diem.) in 1 l of distilled water were steam treated at
100°C during 40 min. After filtration through Whatman
paper no. 1, the liquid fraction and the solid residue
were separated. A previous study showed that the solid
residue was the most appropriate substrate for growth
and aroma production as compared with liquid fraction
and the untreated coffee husk, probably because some
inhibitory compounds (caffeine) present in the coffee
husk were eliminated by the steam treatment [