shrimp. Two days were allowed for the shrimp to hatch and
mature as nauplii (larva). After two days, when the shrimp
larvae are ready, 4 mL of the artificial s
was added to
each test tube and 10 brine shrimps were introduced into each
tube. Thus, there were a total of 30 shrimps per diluti
Then
the volume was adjusted with artificial seawater up to 5 mL per
test tube, The test tubes were left uncovered under the lamp.
The number of surviving shrimps were counted and recorded
after 24 hours.Using probit analysis, the lethality concentration
(LC50) was assessed at 95% confidence intervals LCso of less
than 100 ppm was considered as potent (active) .As mentioned
by Meyer and others, LC50 value of less than 1000 ug/mL is
toxic while LC50 value of greater than 1000 Hg/mL is non-toxic.
N The percentage mortality (%M) was also calculated by dividing
the number of dead naupolii by the total number, and then
multiplied by 100%. This is to ensure that the death (mortality)
of the nauplii is attributed to the bioactive compounds present in
the plant extracts