Methodology
Plant materials and sample preparation
M. alba samples were collected from faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand (April 2011).
Young twigs of M. alba were rinsed with tap water, then sterilized with 20 and 10% sodium hypochlorite for 15 minutes, respectively. After that the explants were washed with sterilized distill water several times and plated on MS medium. Root culture was obtained from root part of the M. alba cultures on half strength MS liquid medium supplemented with 1 mg/l NAA.
Dried powdered of plant samples (10 mg) were weighted, extracted with methanol 500 μl and then sonicated for 15 min. The extracts were centrifuged at 3,000 rpm for 3 min to collect the supernatant. This extraction procedure was repeated four times. The combined extract were evaporated at 50°C and re-
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