In addition to deliberately adulterated model meatballs, five different brands of commercial beef meatballs
(Table 2) under pure, raw and heat treated conditions were blindly tested (Fig. 3). The positive targets were detected only in model meatballs with 1% target adulteration but no contamination was found in commercial meatballs, suggesting the halal sanctity of Malaysian meatballs. This was not surprising since Malaysian government is dedicated to develop ‘‘Halal Hub’’ and has been strictly monitoring markets time to time. In addition to pentaplexing, the assay could be used in simplex, duplex, triplex, tetraplex and multiplex PCR systems to detect the targeted species even from degraded specimens under complex matrices.
In addition to deliberately adulterated model meatballs, five different brands of commercial beef meatballs(Table 2) under pure, raw and heat treated conditions were blindly tested (Fig. 3). The positive targets were detected only in model meatballs with 1% target adulteration but no contamination was found in commercial meatballs, suggesting the halal sanctity of Malaysian meatballs. This was not surprising since Malaysian government is dedicated to develop ‘‘Halal Hub’’ and has been strictly monitoring markets time to time. In addition to pentaplexing, the assay could be used in simplex, duplex, triplex, tetraplex and multiplex PCR systems to detect the targeted species even from degraded specimens under complex matrices.
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