2.2. Preparation of inocula and fermentation experiments
Single colonies of isolates were grown overnight in 50 mL YPS
proliferation media placed in 200 mL bottles. Cultures were performed in thermostatic baths (model G-76, New Brunswick Scientific Co., Edison, NJ, USA) at 30
C and 200 rpm.
The ability to produce ethanol was assayed by triplicate after
inoculation of 50 mL YPS fermentation medium with 0.50 g/L
biomass in 200 mL bottles. Fermentations were assayed during
144 h in oven at 30
C.
Fermentation trials to study the influence of temperature on
three selected strains (A2, A10 and A11) were carried out by triplicate after inoculation of 50 mL YPS fermentation medium with