Endophytic fungi isolated from chilli in Bogor are Aspergillus favus, Coniothyrium sp., Nigrospora sp., sterile hypha 1 (SH1) and sterile hypha 2 (SH2). Colonization of some endophytic fungi has important role in the protection of chili plants against Aphis gossypii. Some of those fungi i.e SH1, SH2, and Nigrospora sp. are able to increase resistance chili against A. gossypii, in which SH2 has the strongest effect. This is play an initial basis for using fungal leaf endophytes as biocontrol agent against pests of chili. Better understanding on the aspects related to endophytic fungi of chili leaves, such as mechanism involve, type of produced toxin, mode of transmission, spectrum of affected insect pests, host-environment relation, should be furthermore elaborated to obtain appropriate strategy and technique for the use in biological control.