The PFGE system has been used to electrophoretically karyotype (EK) yeast through the separation of intact chromosomal DNA (33), but it has limitations in its ability to differentiate C. albicans isolates when compared to restriction analysis (34). To further delineate strains of Candida species, restriction endonuclease analysis of the genome (REAG) using either BssHII or SfiI as the restriction enzyme has proven to be a reliable method with high discriminatory abilities