The purpose of this paper is to develop e-learning based on animation content to improve mathematical connection abilities in senior high school students. The e-learning was developed using framework proposed by Moddle, while the animation content was developed using macromedia flash. To get the student mathematical connection abilities, pretest and postest were administered before and after teaching and learning process. The data were analyzed using t-test and found that e-learning which was based on animation content not only had significant effect on mathematical connection abilities but also been able to improve students’ mathematical connection abilities far better than that of conventional approach.