Microbial levansucrase is formed and secreted in to the extracellular environment at an acidic pH (Venugopal, 2011). The enzyme levansucrase acts highly on sucrose and has low activity on other sugars like fructose, mannose, raffinose, maltose etc. Studies on the activity of levansucrase on different sugars and sugar derivatives showed that a few sugars and sugar alcohols inhibit the action of this enzyme. But the activity of the enzyme is relatively increased when used with inhibitory sugars in the presence of polyethylene glycol (Avineri-Shapiro & Hestrin, 1945; Han, 1990).