Incidence of AFM1 in raw milk was 38.8% and, in all samples, AFM1 levels were lower than the Southern Common Market (MERCOSUR) Regulation (maximum level accepted ¼ 0.5 mg L1). Commercial feed consumption (OR ¼ 4.630, P ¼ 0.001), soybean expeller consumption (>0.95 kgDM/cow) (OR ¼ 3.542, P ¼ 0.019), and cotton seed consumption (>1.5 kgDM/cow) (OR ¼ 2.949, P ¼ 0.089) were associated with the incidence of AFM1 in raw milk. Despite the incidence and the level of AFM1 in milk produced and commercialized in Argentina is not a serious problem for public health.