To determine the effect of the fungicides on mycelial
dry weight of R. solani, potato dextrose (PD) broth was
used. Requisite quantity of each fungicide was added to
the broth to have concentrations of 100, 250 and 500 ppm.
Three replicated flasks were used for each dose of the
three fungicides. The flasks were inoculated with mycelial
discs of 5-day-old R. solani cultured on PDA. These
mycelial disc were cut and put into the flask. Additional
three flasks containing the PD broth receiving no
fungicides were used as controls. The inoculated flasks
were incubated at room temperature (25–28 8C) for
14 days. At the end of the incubation, the cultures in all
flasks were filtered separately through pre-weighted filter
paper. The dry weight of mycelium was determined after
drying the mycelium on filter paper in an oven at 70 8C for
12 h. The dry weight of mycelium was obtained by
subtracting the weight of the filter paper from the
combined weight of the filter paper and of mycelium.
Inhibition of mycelia dry weight was measured comparing
with the control flasks.