Tiliacora triandra has long been used as a traditional medicine and in cuisine in Thailand and neighboring countries. Leaf samples were randomly collected and extracted with methanol. The extracts were chemically analyzed by gas chromatography-mass spectrometry (GC-MS), and free radical scavenging activities were assessed by the 2,2-diphenyl-1picrylhydrazyl (DPPH) assay. After drying and re-dissolving the extracts in dimethyl sulfoxide (DMSO), cytotoxicity and genotoxicity tests were performed using lymphocytes and HeLa cells. The major components found in all extracts, with yields of 8.59-26.29%, were vitamin E, phytol and 1-cyclohexenylacetic acid. At a concentration of 8.4 mg/ml, the methanol extract reduced the concentration of free radical DPPH (DPPH•) to 50% (EC50). Lymphocyte viability was 72.78% when treated with 10 mg/ml extract, a concentration that significantly affected DNA (p