No studies have compared the effects of static stretching (SS) with motor imagery on anaerobic performance in trained cyclists. The purpose of this study was to examine the effects of static stretching compared with motor imagery and quiet rest (QR) on anaerobic performance in trained cyclists. Thirteen trained cyclists (9 men: 4 women; aged 21 ± 2 years) were assessed for height (1.76 ± 0.07 m), weight (73.4 ± 13 kg), % body fat (10.8 ± 6.2%), and maximal oxygen consumption ( VO2max of 42.0 ± 5.6 ml_kg_min) on a cycle ergometer.