The aim of this study is to characterise the genotype of 19 isolates of vetiver obtained from Hydromulch (Pty) Ltd via sequencing analyses of three DNA fragments, ITS, ndhF and rbcL. According to the results generated during this study very little or no genotypical differences exist amongst the different isolates available from the Hydromulch (Pty) Ltd plant collection. Only in the case of the ITS inference were differences observed between 3 isolates.