Fresh C. crepidioides leaves were obtained from a local
farmland in the Akure metropolis, Nigeria. The sample was
authenticated at the Department of Crop, Soil and Pest
Management, Federal University of Technology, Akure,
Nigeria. The inedible part was removed and thoroughly
washed with distilled water to remove any dirt, chopped into
small pieces using a table knife and immediately transferred
into a vessel containing tap water to prevent oxidation. Then,
the samples were randomly divided into two (2) portions.
One portion was blanched for 10 min at 80 C, while the
other portion was not. The blanched portion was then
drained. Both portions were dried in hot air at 40 C and
then milled into a powder using a laboratory blender. One
gram of each powdered sample was extracted with 100 mL of
methanol and 1 N HCl (1:1 w/v). The filtrate was then
evaporated to dryness using a rotary evaporator. The flask
containing the extracts was thoroughly washed with distilled
water and then frieze dried with the aid of a freeze drier. One
hundred milligrams of each dried extract were then reconstituted
in one hundred millilitres of water and stored in a
refrigerator for subsequent analysis. The freeze-dried samples
were used for the GC-FID analysis.