In this study, bioethanol was successfully produced from lipidextracted biomass of S. dimorphus with maximum bioethanol yield of 0.26 g bioethanol/g microalgae (95.59%) equivalent to 7.34 g/L in 2 h. Result shows that the experiment using S. cerevisiae works well in the SSF environment. It was able to produce signifi- cant bioethanol yield with an overall value of more than 80%. Maximal ethanol yield was identified at yeast concentration of 3 g/ L, pH 5, 36 C and enzyme concentration of 60 units/mL. This study is essential to provide the basis of knowledge to produce biofuel as commodity products using biorefinery concept. In light of this, more detail study are needed for production of binary biofuel with the aim to sustain energy and cost efficiency.