The activity ofMDA in whole blood samples was determined using a
lipid peroxidation MDA assay kit according to the manufacturer's
instructions. The binding of thiobarbituric acid to malondialdehyde,
which was formed during lipid peroxidation, results in a chromogenic
complex. In the spectrophotometer, the change of absorption
peak was detected at 532 nm. Colorimetry was used to detect the
malondialdehyde activity in the whole blood samples. Additionally, the
activity of GSH was determined using the GSH assay kit. The GSH
activity was determined by the reaction of GSH with 5.50-dithiobis-2-
nitrobenzoic acid (DTNB) to produce a product that could be measured
using a spectrophotometer at 412 nm.