Total phenolic contents were determined according to Lachman et al.[16] 1mL of sample
extract was transferred into a 50mL volumetric flask and diluted approximately with 5mL
distilled water. Then, 2.5mL Folin-Ciocalteau reagent and 7.5mL of 20% (w/w) Na2Co3 were
added and made up to 50mL with distilled water. It was agitated and left to stand for 2hrs.
Absorbance of the sample was measured on the spectrophotometry at 765nm against a blank
prepared with distilled water. Gallic acid was used for calibration. The results were expressed
as Gallic acid equivalent (GAE) in g/100g.