which has been previously described [21]. Briefly, the HSH method consists of the production of NLCs by preparing two different surfactant phases, lipid and aqueous obtained separately. The lipid phase consisted of CP, GS and GSO or HPO or SBO and the aqueous surfactant phase of Synperonic F68, Tw20 or Tw80, lecithin and double-distilled water. They were heated to the same temperature of 85 8C for 30 min. To the lipid phase, various amounts of GTE were added. Before mixing the two phases, the aqueous surfactant phase was stirred for 2 min at high speed (15 000 rpm). Then, a pre-emulsion was produced by high shear stirring and processed by HSH. Trehalose at the concentration of 5 wt% was used in the freeze-drying process as the cryoprotector. NLCs suspensions were frozen in an aqueous trehalose solution at –25 8C for 24 h, and then, the samples were transferred to the freeze-dryer at –55 8C for 72 h. After that, the NLCs powders were collected for further experiments. The obtained lyophilized samples exhibit a powdery aspect.