Eucalyptus (Eucalyptus sp.) young-propagated cuttings grown under commercially nursery production have been annually affected by wilt disease. The 187 bacterial strains isolated from symptomatic seedlings (stem, leaves, root, soil) were identified as Ralstonia solanacearum using morphological, physiological and biochemical characterization including 16s rDNA and pathogenicity analyses. These strains induced a HR on tobacco (cv. xanti) within 24 h, where the signs of wilting (yellowing, chlorosis, leaves drop) were observed on E. grandis (cv.TD-04) at 5-15 days after inoculation by root dipped into the bacterial suspension (108 cfu/ml). They were further subdivided into races and biovar that the 7 strains from eucalyptus showed virulence on tobacco (cv. xanti) and infected tomato, chilli, eggplant, Thai eggplant, hot pepper, pepper, turkey berry and marigold but did not affect either potato or ginger.