Fish were fed to satiation twice daily at 0900 and
1800 for 20 days. A pre-weighed ration was provided
to each tank, and if completely consumed additional
pellets were counted out and provided until feeding
ceased. Any uneaten pellets were siphoned out after
10 min and counted in order to determine total daily
food consumption. On day 20, fish from one replicate
from each of the four temperature treatments were
starved for 24 h. Following this starvation period,
individual weight (g) and total length (mm) were
measured for all fish. Five fish from each tank were
killed with an overdose of benzocaine (400 mg l1),
autoclaved and freeze-dried to a constant weight in
order to determine whole-body chemical composition.
On days 21 and 22 fish from the remaining two
replicates of each treatment were starved for 24
h and sampled as described above.