Ethanol is a biomass which is a cleaner fuel. It can burn completely so, it reduces air pollution and global warming. Ethanol was produced by raw materials from agricultural crops. It classified into three types include sugar (sugar from beets and molasses), starch (tapioca, potatoes, and whole grains) and byproducts from agriculture (bagasse, rice straw, and corn). In the production of ethanol, it causes wastewater which has BOD and COD about 40000-70000 and 100000-150000 mg/l, respectively. Moreover, wastewater has high nitrogen and phosphate concentration and has dark brown to black. Those things affect to water quality and scenery of water source thus, it cannot be released into water bodies.