MATERIALS AND METHODS
Collection of samples
The sample fishes used in this work were collected from the local water bodies. The carp, Catla catla (1–2 kg) and Labeo rohita (200–400 gm) were chosen as per availability in the market for the study. The bile was collected from intact gall bladder of two different fish species separately. The collected bile was then preserved at 0ºC for further study.