4.2. Quantitative determination of reducing sugar using Benedict’s
solution
The titration of 1% (0.25 ml) glucose standard with Benedict’s
solution required 0.018 ml of the sample. This gave a 13.9%
concentration of reducing sugar. This concentration was diluted to
10% before fermentation and the rate kinetics was studied.
From the kinectics of the fermentation, it was noticed that up to
60% reducing sugar was used up in the
first 8–10 h of the process as
shown in Table 2 and Fig. 2.