In briefly,a spore suspension at 1.0 × 106 spores/mL(100 L) was dispersed evenly with a sterile spatula on PDA medium supplemented with or without 0.6% NaHCO3. After 2, 4 and 6 days of incubation at 25◦C, ten plugs (5 mm diameter) of mycelial agar (about 10 g) were obtained randomly and immersed into 10 mLethyl acetate for 1 h. After centrifugation