Immunocapture was conducted following the method described by Jacobi [13]. Briefly, 0.2 mL
thin-wall polypropylene PCR tubes in strips were coated with 30 μL of 1.0 μg/mL anti-TMV antibody
(ADGEN Diagnostic Systems) in ELISA coating buffer and incubated for 2–4 h at 37 °C or overnight
at 4 °C. The supernatant of soil suspension or plant sap was loaded in the anti-TMV antibody coated PCR tubes and inoculated for 2 h at 37 °C